Core Equipment Available at MetroHealth Research Institute

Advanced Flow Cytometry Core

Sony ID7000 Spectral Cell Analyzer - Sony

The Sony ID7000 Spectral Cell Analyzer is a high-parameter spectral cytometer. This system is configured with 5 lasers (355, 405, 488, 561 and 637nm) and 147 detectors. It enables researchers to perform experiments using 44 fluorochromes.

Sony MA900 Multi-Application Cell Sorter - Sony

The Sony MA900 Multi-Application Cell Sorter is a cell sorter with 4 excitation lasers (405, 488, 561 and 638nm). This instrument can detect up to 12 fluorescence parameters and two scatter parameters. The MA900 software guides operation, making it very easy for users to learn and use.

CytoFLEX Flow Cytometer - Beckman

The CytoFLEX is a flow cytometer equipped with 3 active lasers (405, 488 and 638nm) and 13 channels for fluorescence detection.

Guava Muse Cell Analyzer – Cytek Biosciences

The Cytek Guava Muse Cell Analyzer is a user-friendly flow cytometer that allows user to perform a broad range of assays. The Guava & Muse assays available are Count and viability, Cell proliferation, Apoptosis, Cell signaling, Cell cycle, DNA damage, Autophagy, Immunology and Malaria research.

GentleMACS Octo Dissociator with Heaters - Miltenyi Biotec

The gentleMACS Octo Dissociator with Heaters is a benchtop instrument for tissue dissociation or homogenization of up to eight biological samples. This instrument allows the user to obtain single-cell suspensions or thorough homogenates in a closed and sterile system.

Light Microscopy Core

Thunder Assay Microscope System - Leica

The Thunder Assay Microscope System contains a microscope for color and fluorescence imaging. This instrument is configured with 8 lasers (390, 440, 475, 510, 555, 575, 635 and 747nm), which allows the user to perform fluorescence imaging. It is also equipped with a colored camera that can image histology slides (ex: H&E). The Thunder Imaging System enables users to obtain a clear view of details, even deep within an intact sample, in real time without out-of-focus blur.

Thunder Assay Workstation - Leica

Data processing with Avia AI analysis system

TCS SP8 Scanning Confocal Microscope - Leica

Live cell imaging

Eclipse Ts2R Inverted Research Microscope - Nikon

The Eclipse Ts2R is an inverted microscope for wide-field fluorescence (385, 475, 550 and 621nm) or phase contrast on live cell or tissue section imaging.

Eclipse Ts2 Inverted Research Microscope - Nikon

The Eclipse Ts2 is an inverted microscope for wide-field fluorescence (470 and 560nm) on live cell or tissue section imaging.

Advanced Cell Biology Core

Incucyte S3 - Sartorius

The IncuCyte S3 is a live cell analysis system that can image and analyze cell cultures inside an incubator. It allows real-time monitoring of various cell-based assays (from cell health to complex functional assays) through the utilization of its fluorescence and phase imaging channels simultaneously.

4D Nucleofector X Unit - Lonza

The 4D-Nucleofector X Unit supports nucleofection of various cell numbers (2x10^4 to 2x10^7). All cell type-specific optimized protocols are available in the Lonza database.

Small Animal Imaging Core

IVIS Lumina X5 - Revvity

The IVIS Lumina X5 is an advanced imaging system deisgned for in vivo imaging. It has imaging capabilities for bioluminescence, fluorescence, color and x-ray.

Exosome Core

ZetaView – Particle Metrix

The ZetaView is a nanoparticle tracking analyzer that can scan and monitor various types of bio-nanoparticles, such as extracellular vesicles, exosomes and viruses.

Molecular Biology and Biochemistry Core

Spectra Max ID3, Multi-Mode Microplate Readers - Molecular Devices

The SpectraMax iD3 is a multi-mode microplate reader designed to measure absorbance, fluorescence, and luminescence in microplates. This instrument can also measure time-resolved fluorescence and fluorescence polarization.

Nanodrop Microvolume Spectrophotometers - Thermo Fisher

The Nanodrop provides a simple, rapid and accurate qualification and quantification of DNA, RNA and protein samples.

GelDoc Go Imaging System - BioRad

The GelDoc Go Imaging System allows users to acquire images of both nucleic acid and protein gels.

QuantStudio3 Realtime PCR System – Applied Biosystem

The QuantStudio3 is a real-time PCR system that is designed to be affordable and user friendly.

Azure 600 - Azure Biosystems

The Azure600 offers two channels (Red and Green), laser-based infrared and chemiluminescent detection. It allows users to image visible fluorescent dyes, standard ethidium bromide and protein gels, and excitation for quantitative Western blot imaging.

If you would like more information on any of this equipment please contact:

Kevin Cheng - Research Core Facilities Manager: [email protected] or Roger Shi, PhD - Core Facility Diretor: [email protected]